Jing Cheng Nanotechnology Research Center of Stem Cells and clinical cooperation against JM nanometer new composite material against viruses following tests:
Using a transparent spot forming region (Plaque Forming) method, the virus was observed by JM material effect after the residual amount of the culture broth.
Virus Type:
1. Enterovirus LLC-MK2: Enterovirus UNIKOM eleven type, from the College of American Pathologists Proficiency Test strains.
2. Type A influenza virus MDCK: A type influenza virus New Caledonia / 20/99 (H1N1), from the College of American Pathologists Proficiency Test strains.
Host cell:
1.A type influenza viruses using MDCK cell lines (BCRC 60004).
2. Enterovirus using LLC-MK2 cell line (BCRC 60092).
JM Nano new composite materials TTA anti-virus test:
The test virus enterovirus species were associated with influenza A virus, when cells are infected with the virus, the cells die (as shown below), and then crystallized by MTT staining pattern test for interstitial cell enzyme activity by the effect of the order analysis of cell viability was used to determine the antiviral effect, when the material for the test of the effectiveness of antiviral higher the higher cell viability, but due to different viruses and bacteria can not be measured directly, so the cells infected with a virus plaque formation ( plaque) of the method to calculate the virus plaque forming units (PFU; plaque-forming unit) to determine the concentration of virus in units of PFU / Ml.
Using a transparent spot forming region (Plaque Forming) method, the virus was observed by JM material effect after the residual amount of the culture broth.
Virus Type:
1. Enterovirus LLC-MK2: Enterovirus UNIKOM eleven type, from the College of American Pathologists Proficiency Test strains.
2. Type A influenza virus MDCK: A type influenza virus New Caledonia / 20/99 (H1N1), from the College of American Pathologists Proficiency Test strains.
Host cell:
1.A type influenza viruses using MDCK cell lines (BCRC 60004).
2. Enterovirus using LLC-MK2 cell line (BCRC 60092).
JM Nano new composite materials TTA anti-virus test:
The test virus enterovirus species were associated with influenza A virus, when cells are infected with the virus, the cells die (as shown below), and then crystallized by MTT staining pattern test for interstitial cell enzyme activity by the effect of the order analysis of cell viability was used to determine the antiviral effect, when the material for the test of the effectiveness of antiviral higher the higher cell viability, but due to different viruses and bacteria can not be measured directly, so the cells infected with a virus plaque formation ( plaque) of the method to calculate the virus plaque forming units (PFU; plaque-forming unit) to determine the concentration of virus in units of PFU / Ml.
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